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. 2014 Oct 16;10(10):e1004464. doi: 10.1371/journal.ppat.1004464

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A. Predicted mRNA target sequences. Targets for slmiR482f and slmiR5300 were identified using the Sol Genomics website (http://solgenomics.net). Alignments were made using ClustalW2. The nucleotides shown in red in each mRNA target are mismatches with the corresponding miRNA. B. Protein domains. Analysis was performed using Interpro (http://www.ebi.ac.uk/interpro/). NB: A signaling motif shared by plant resistance gene products and regulators of cell death in animals. CC: Coiled-Coil domain. LRR: Leucine-rich repeats. DUF3542: Protein domain of unknown function found in eukaryotes and viruses. The NB domain in Solyc05g008650 is truncated at the C-terminus. The red arrow indicates the miRNA binding site in the P-loop region of the NBS-ARC domain.