Figure 6. Inclusions in rMARV_PSAPmut–infected cells are more densely packed with nucleocapsids than inclusions in rMARV_wt–infected cells.

Huh-7 cells were infected with rMARV_wt or rMARV_PSAPmut. At 28 h p.i., cells were processed in two ways (i) fixed, scraped, pelleted and then embedded in Epoxy resin (A and C); or (ii) fixed and embedded in Epoxy resin on Thermanox slides (B and D). Ultrathin sections were stained with uranyl acetate and subjected to electron microscopy. (A–B) rMARV_wt–infected cells, (C–D) rMARV_PSAPmut–infected cells. Bars, 500 nm. (E) Morphometric analysis of inclusions. Volume density of nucleocapsids inside inclusions is shown, p-value (***, p≤0.0001). (F) Amount of electron dense (mature) nucleocapsids inside inclusions (see black arrows Fig. 6B und D) determined per 2.5 µm² of inclusion at electron micrographs, p-value (*, p≤0.05).