Figure 2. Transient expression of specific myosin tails in the inoculated leaf delays or inhibits the onset of systemic spread of TMV-GFP-JL24.

A, the onset of systemic spread is delayed upon transient expression of myosin tails XI-2, VIII-B, VIII-1 and VIII-2 in the inoculated leaf (arrow), whereas expression of XI-F tails has no effect. Overexpression of myosin tails XI-K inhibits the systemic movement of TMV. Images are representative for the results obtained in three independent experiments, each with four plants for each treatment. Single leaves of each plant were inoculated with equal amounts of TMV-GFP-JL24 virions and agroinfiltrated for myosin tail/RFP expression two days later. Plants were scored for systemic infection at 6, 7 and 8 dpi. B, percentage of systemically infected plants at 6, 7 and 8 dpi.