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. 2014 Oct 16;10(10):e1004448. doi: 10.1371/journal.ppat.1004448

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© 2014 Amari et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, co-expression of MP:GFP with RFP. The MP:GFP accumulates in PD. B and C, co-expression of the MP:GFP together with myosin XI-2 tails does not affect significantly the localization of the MP:GFP to PD (B) but causes the accumulation of the MP:GFP in a single big aggregate in the vicinity of the nucleus (C, arrow). D, transient co-expression of myosin XI-2 tails and MP:RFP in N. benthamiana transgenic plants expressing GFP:HDEL as ER marker (16c). Accumulation of the MP:RFP in a single aggregate near to the nucleus upon inactivation of myosin XI-2 correlates with the accumulation of the GFP:HDEL indicating that the MP aggregates are ER-derived. E, co-expression of the MP:GFP with myosin XI-F tails. The MP:GFP accumulates in PD similar as in the control with RFP (A). F and G, co-expression of the MP:GFP together with myosin XI-K tails has no significant effect on the localization of the MP:GFP to PD (F) but causes accumulation of the MP:GFP in several aggregates (G, arrow). H, immunoblot analysis using HA-specific (top panel) and GFP-specific (middle panel) antibodies for the detection of myosin and MP:GFP, respectively. Bands corresponding to the class XI (≈100 kDa) myosin tails are marked by asterisks. Coomassie blue staining (bottom panel) is shown as loading control. A-H, proteins were expressed by co-agroinfiltration and observed at 1 dpa. N, Nucleus. No, MP:GFP expressed alone. Scale bars, 20 µm (A-C and E-G) and 10 µm (D).