Figure 4.

Effect of sonodynamic therapy (SDT) on apoptosis detected by TUNEL assay. The images were taken at 24 hours after treatment with SDT (A), ultrasound (US) (B), and hematoporphyrin monomethyl ether (HMME) (C), or after no treatment (D). Summary of in situ apoptosis assay at different times (24 hours, 3 days, and 7 days) after the indicated treatments (E).

Notes: Data are presented as the mean ± standard deviation (n=8); *P<0.01 versus control. Apoptotic cells are indicated by the arrows.

Abbreviation: TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.