Figure 5.

Effect of sonodynamic therapy (SDT) treatment on the expression of protein cytochrome-c (Cyto-C) and protein caspase-3. Immunohistochemical staining with Cyto-C antibody at 24 hours after treatment with SDT (A), ultrasound (US) (B), and hematoporphyrin monomethyl ether (HMME) (C), or after no treatment (D). Summary of the effect of SDT on protein Cyto-C (E). Immunohistochemical staining with caspase-3 antibody at 24 hours after treatment with SDT (G), US (H), and HMME (I), or no treatment (J). Summary of the effect of SDT on protein caspase-3 (F).

Notes: Data are presented as the mean ± standard deviation (n=8); *P<0.01 versus control. Positively stained cells are indicated by arrow.