Figure 2. (A) H&E staining of skin showing epidermis (a). Y -FISH on Caesarean scar of women with previous male pregnancy showing male cells in epidermis (b) (arrows). H&E staining of skin showing dermis (c) and male cell in dermis (d) (arrows). Arrows (yellow; aandc) demonstrate putative location of these cells in H&E stained sections. Male cells identified in the female caesarean scar, bearing a Y chromosome labeled with SpectrumOrange and blue represents DAPI stained nuclei. (B) (a) H&E staining of skin showing blood vessels in dermis and (b) XY-FISH showing a male cell around a blood vessel in dermis, Male cell bearing an X chromosome labeled with SpectrumOrange and a Y chromosome labeled with SpectrumGreen. Green in the center of blood vessel is autofluorescence caused by blood. All chromosomes may not appear in the same plane of focus due to the thickness of sections (5 µm).