Figure 4. Combined FISH and immunostaining of Caesarean scars from pregnant women. All 100x. (AandB) Collagen I positive male cells in epidermis. (CandD) Collagen III positive male cells in epidermis. (E) TGF-β3+ male cell bearing a Y chromosome (red). (F) TGF-β3+ male cell with X chromosome labeled with SpectrumOrange and Y chromosome labeled with SpectrumGreen. (G) Fibronectin+ male cell bearing a Y chromosome (red) in dermis. (H) Fibronectin+ male cell (XY) in epidermis of skin. In A, C, E, andG, blue represents the DAPI staining of nuclei and green is staining of antibody conjugated with fluorescein Streptavidin, whereas in B, D, F, andH, blue represents staining of antibody conjugated with fluorescein AMCA