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. 2001 Nov 30;70(1):83–100. doi: 10.1086/338458

Figure 5.

Figure 5

PCR analysis of a 550-kb duplication between human chromosomes 14 and 22. The figure shows the position on chromosome 22 of oligonucleotides that were designed to amplify paralogous sequences from chromosomes 14 and 22. Ten PCR products (A-K) were designed, spanning ∼550 kb. Products were amplified and sequenced from both chromosome 14 and chromosome 22. The total high-quality sequence (SEQ TOTAL), the number of sites with fixed differences (SEQ fixed) between 22 and 14, and the number of heterogeneous sites (SEQ hetero) for each product are shown. These heterogeneous sites in the most centromeric products suggest multiple copies for this region within chromosome 14. The average sequence identity between chromosome 14 and 22 for all 3,215 bases was 99.4%–99.8% (with and without heterogeneous sites).