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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: Clin Cancer Res. 2014 Aug 27;20(20):5255–5264. doi: 10.1158/1078-0432.CCR-14-1019

Figure 2.

Figure 2

CPI-613 inhibits mitochondrial respiration. A) Oxygen consumption rates (OCR) are shown for K562 and OCI-AML3 cells treated with CPI-613. Cells were incubated with the indicated concentration of CPI-613 for 2 hours prior to the assay. The electron transport chain uncoupler FCCP was added and OCR was measured in triplicate from 3 separate measurements, repeated in 3 independent experiments. Error bars are the standard deviation. *=p value <0.05. B) PDH phosphorylation is increased by CPI-613. K562 cells were incubated with the indicated amount of CPI-613 as in A and harvested for lysates. Western blots for phospho-E1α were performed. Actin was blotted to serve as a loading control. C) AMPK phosphorylation is increased by CPI-613. OCI-AML3 cells were incubated with the indicated amount of CPI-613 as in A and harvested for lysates. Western blots for phosphorylated AMPK (pAMPK) were performed. Total AMPK (tAMPK) was blotted to serve as a loading control.