Induction of the STAT-Socs pathway in whole liver after injection of erythropoietin (rhEPO). A. Immunoblotting for phosphoSTAT5 and phosphoSTAT3 whole liver after rhEPO injection. Total STAT5 and total STAT3 are shown as loading controls. B, C. demonstrate densitometry analysis of the immunoblots shown in A, n=3–5 per time point. *=p<0.05 versus untreated. D. RNA was isolated from whole liver after rhEPO injection, and expression of Cis, Socs1, and Socs3 was assessed by qPCR. rhEPO significantly induced Socs3 expression 90 minutes after injection. * = p<0.05