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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Shock. 2014 Nov;42(5):424–431. doi: 10.1097/SHK.0000000000000231

Figure 5.

Figure 5

Induction of protective pathways in isolated hepatocytes. AML12 cells were stimulated with rhEPO and cell lysates were prepared. Immunoblotting revealed that rhEPO does not activate STAT5 (A) or STAT3 (B) in hepatocytes in vitro. Total STAT5 and total STAT3 are shown as loading controls, and densitometry analysis of n = 3–5 samples per time point is shown. C. rhEPO did not induce expression of Socs1 or Socs3 in AML12 cells, as assessed by qPCR. D. rhEPO induces HO-1 expression in hepatocytes in vitro. B-actin is shown as a loading control, and densitometric analysis of n=3–5 samples per time point is shown.