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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: Cancer Res. 2014 Aug 27;74(20):5914–5924. doi: 10.1158/0008-5472.CAN-14-0834

Figure 6. Identification of conserved direct targets of ZFX in human medulloblastoma cells.

Figure 6

(A) Downregulated genes (n= 163) identified in pattern matching analysis of Affymetrix Gene ST 1.0 array data from DAOY cells after shRNA knockdown. Full gene list is presented in Dataset S1.

(B) Conserved ZFX transcriptional targets. Shown are numbers of pattern-matching genes from ZFX KD microarray in (A) that had enrichment peaks >1kb from TSS in ZFX ChIP-seq from DAOY cells, from murine embryonic stem cells (mESC), from both cell types (Overlap), or from neither cell type (N/A). DAOY, mESC, and Overlap gene lists are presented in full in Dataset S2.

(C) Zfx binding to Dis3L in murine and human ChIP-seq. Shown are sequencing-read enrichment peaks near TSS of Dis3L in anti-Zfx ChIP-seq from murine ESC (Chen et al., 2008) (left), and in anti-ZFX ChIP-seq and sheared nuclear lysate control (Input) from DAOY human MB cells (right).

(D) Zfx binding to Ube2j1 in murine and human ChIP-seq. Shown are sequencing-read enrichment peaks near TSS of Ube2j1 in anti-Zfx ChIP-seq from murine ESC (Chen et al., 2008) (left), and in anti-ZFX ChIP-seq and sheared nuclear lysate control (Input) from DAOY human MB cells (right).