(A, D) IMR-90, 267B1 and K-ras transformed 267B1 (267B1/K-ras) cells were transfected with siRNAs as indicated. At 3 days after transfection, cell lysates were analyzed by immunoblotting.
(B, E) 72 hours after siRNA transfection, cellular ATP levels were measured (mean ± SD; n = 3). *** p< 0.001
(C, F) To measure cell growth, 2.0 × 105 cells transfected with the indicated siRNAs were plated. After 72 hours, the number of cells was counted (mean ± SD; n = 3). *** p < 0.001