Skip to main content
. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: J Cell Biochem. 2014 Dec;115(12):2165–2174. doi: 10.1002/jcb.24893

Figure 4. RNPS1 sub-nuclear localization is influenced by Acinus isoforms.

Figure 4

A. RNPS1 and Acinus isoforms co-localize in the nucleus. B. The C-terminal RS- and RD/E-rich region of Acinus is necessary for its co-localization with RNPS1. COS7 cells were transfected with one of the following GFP-tagged Acinus isoforms [wild type Acinus-S′ (A), wild type Acinus-L (A), SAP-Acinus-S′ (B) or SAP-Acinus-S (ΔC) (B)] and 3XFLAG-tagged RNPS1 (A and D) expression plasmid DNAs at a ratio of 6:1.. Twenty-four hr after transfection, cells were fixed and Acinus proteins were directly visualized using fluorescent microscopy. RNPS1 was detected by immunofluorescent microscopy following staining with anti-FLAG primary antibody and rhodamine conjugated second antibody. V5 empty vector DNA was used as the control. DAPI was used as a nuclear stain. Scale bar equals 10 μm.