Table 2.
Effect of TBCA on mitochondrial membrane potential in PC3-LN4 cells over time
| Time of treatment (h) | Agent | Mitochondrial membrane potential (Δψm) in PC3-LN4 cellsa | ||
|---|---|---|---|---|
| High Δψm (red) |
Low Δψm (yellow) |
No Δψm (green) |
||
| 2 | None | 86.3 ± 1.3 | 13.8 ± 1.3 | 0 |
| DMSO | 90.5 ± 0.9 | 7.9 ± 0.8 | 1.6 ± 1.0 | |
| 20 μM TBCA | 35.3 ± 3.4 | 29.3 ± 1.4 | 34.3 ± 1.9 | |
| 80 μM TBCA | 20.3 ± 2.4 | 26.6 ± 1.0 | 53.1 ± 2.1 | |
| 4 | None | 90.8 ± 1.2 | 4.4 ± 0.8 | 3.0 ± 1.1 |
| DMSO | 89.2 ± 1.0 | 10.3 ± 0.7 | 0.7 ± 0.1 | |
| 20 μM TBCA | 40.9 ± 5.8 | 36.0 ± 3.7 | 23.0 ± 3.6 | |
| 80 μM TBCA | 14.0 ± 2.1 | 31.3 ± 2.0 | 54.6 ± 5.8 | |
| 6 | None | 88.8 ± 2.6 | 10.1 ± 2.0 | 1.0 ± 0 |
| DMSO | 91.4 ± 1.1 | 7.5 ± 1.2 | 1.3 ± 0.5 | |
| 20 μM TBCA | 88.5.0 ± 2.7 | 9.6 ± 2.1 | 1.9 ± 0.5 | |
| 80 μM TBCA | 13.5 ± 3.1 | 37.1 ± 1.6 | 49.3 ± 2.5 | |
| 24 | None | 90.4 ± 1.0 | 9.6 ± 1.0 | 0 ± 0 |
| DMSO | 94.8 ± 0.3 | 5.3 ± 0.3 | 0 ± 0 | |
| 20 μM TBCA | 17.6 ± 3.6 | 33.5 ± 2.5 | 48.9 ± 1.9 | |
| 80 μM TBCA | 0.6 ± 0.5 | 0 ± 0 | 99.4 ± 1.7 | |
a
The mitochondrial membrane potential Δψm is represented by red (high), yellow (low), and green (none). Data are presented as mean±SEM. The amount of DMSO as the control corresponded to the higher concentration of TBCA.