. Author manuscript; available in PMC: 2015 Dec 1.

Published in final edited form as: J Cell Biochem. 2014 Dec;115(12):2103–2115. doi: 10.1002/jcb.24887

Table 5.

FACS analysis of the effect of TBB on mitochondrial membrane potential in PC3-LN4 cells over time^a

Agent	Time of treatment (h)	Cells with intact Δψ_m (%)	Cells with loss of Δψ_m (%)
None		97.3	2.7
DMSO^b	2	96.6	3.4
1μM TBB	2	97.7	2.3
DMSO^b	2	65.4	34.6
80 μM TBB	2	21.0	79.0
DMSO^b	24	55.3	44.7
80 μM TBB	24	0.3	99.7

Following the various treatments as described under Experimental Procedures, cells were analyzed by FACS and the percentage of cells with intact or loss of Δψ_m was determined by gating on the untreated control cells.

The amount of DMSO as control corresponds to the volume of the TBB solution added.