Figure 7. HMGB1 down-regulates T cell expression of L-selectin.

A, Twenty-nine days after tumor inoculation the MC38 tumor-bearing mice from figure 6A were sacrificed and blood leukocytes were analyzed by flow cytometry for L-selectin expression and compared to blood leukocytes from tumor-free C57BL/6 mice. Representative histograms showing L-selectin expression from gated CD45⁺CD3⁺CD4⁺ and CD45⁺CD3⁺CD8⁺ T cells from tumor-free, A box-treated, or control-treated (PBS) C56BL/6 tumor-bearing mice. B, Average percent ± SD of CD45⁺CD3⁺CD4⁺ or CD45⁺CD3⁺CD8⁺ T cells expressing L-selectin. n= 5 mice/group (PBS-treated and tumor-free groups); n= 3 mice/group (A Box-treated group). p values were obtained by Student's t test. Data are from one of two independent experiments. C, Gr1⁺CD11b⁺ cells from tumor-free (left-hand panels) or tumor-bearing (right-hand panels) mice were incubated in vitro for zero, two, or four 4 hours with exogenous HMGB1 (left-hand panels) or ethyl pyruvate (right-hand panels), and stained for Gr1, CD11b, and ADAM17. Gated Gr1⁺CD11b⁺ cells were analyzed for ADAM17 expression. Graphs represent MCF of ADAM17 on Gr1⁺CD11b⁺ cells. Data are representative of three independent experiments.