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Kinetic parameters of MaTrxR.
| substrate | Km (µM) |
Kcat (min−1) |
Kcat/Km (µM−1 min−1) |
|---|---|---|---|
| NADHa | 736 ± 57 | 49 | 0.067 |
| NADPHa | 6.3 ± 0.5 | 39 | 6.2 |
| MaTrx7b | 86 ± 5 | 70.5 | 0.82 |
Measured using the DTNB assay as described in materials and methods
Measured using the GHOST assay with NADPH as described in materials and methods
