Figure 1. β-catenin is highly expressed in cells with EGFR activating mutations.
(A) Immunoblots of extracts from cancer cell lines harboring wild type or mutant EGFR. (B) Confocal images of A549, NCI-H1650, NCI-H1975 cells stained with anti β-catenin antibody and visualized by Alexa Fluor 488. Scale Bar = 10 µm. (C) Immunohistochemical analysis of lungs isolated from a CCSP-rtTA mouse (I, II, III, IV) and lung tumors from an EGFR-L858R (LR)-T790M (TM) (EGFR-LR-TM)/CCSP-rtTA double transgenic mouse (V, VI, VII, VIII). Both animals were sacrificed after 20 weeks of doxycycline administration. Scale bar = 50 µm.