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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: Cancer Res. 2014 Aug 27;74(20):5891–5902. doi: 10.1158/0008-5472.CAN-14-0184

Figure 6. Deletion of Ctnnb1 impairs tumor formation in vivo.

Figure 6

(A) Scheme of EGFR-TL, Cre, and Ctnnb1construct for conditional transgenic mice. (B–E) Generation of lung specific conditional Ctnnb1knockout / EGFR-L858R-T790M transgenic mice. I: CCSP-rtTA/Ctnnb1 F/+ ; II: EGFRTL/CCSP-rtTA/teto-Cre/Ctnnb1 F/F; III: EGFRTL/CCSP-rtTA/teto-Cre/Ctnnb1 F/+; IV: CCSP-rtTA/teto-Cre/Ctnnb1 F/F. (B) MRI images of transgenic mice treated with doxycycline for 10 weeks. Arrows indicate lung tumors. “H” indicates location of the heart. (C) Lung weight isolated from mice treated with doxycycline for 8–28 weeks. Data represents mean ± standard deviation. * indicates p ≤ 0.01. (D) H&E staining of lungs isolated from mice. Scale bar = 100 µm. (E) Survival curves after administration of doxycycline. P-value was determined by the log-rank test.