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Native chemical ligation to form Ac-αS1-19 V′3 (9). Left: reaction scheme. Right: reaction monitored by HPLC (monitored at 215 nm) and MALDI MS of product (9) peak (m/z Calcd: 2026.0, Found: 2026.3). Asterisk indicates the expected mass of Ac-αS1-19 (oxoamide at position 3).
