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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Mutat Res. 2014 Apr 13;0:53–63. doi: 10.1016/j.mrfmmm.2014.04.002

Fig. 5. Effect of DNA-PK inhibition on NM-induced phosphorylation of H2A.X at Ser139 and p53 at Ser15 in JB6 cells.

Fig. 5

Whole cell lysates were prepared from JB6 cells treated with 10 µM NU7026 1 h prior to 0.75 µM NM exposure for 4 to 24 h. 80 µg protein was subjected to SDS PAGE followed by western blot analysis for H2A.X Ser139 and p53 Ser15 as detailed under Materials and Methods and the densitometry values represented as numbers under the bands were calculated employing NIH ImageJ software.