Table A8.

Primer Pairs Used for Long-Range PCR Amplification of Deletion Junctions^[Note]

Patient	PrimerIdentificationNumbers	Forward Primer	Reverse Primer	Deletion-JunctionProduct Size(kb)
WHT2943	11130/1	GTAATGCCATTGAGGCAAAGTTGAGTTAAAA	CCACACTGACTTGTACAATGGCCGAA	6.7a
WHT3410	11126/7	GGCCTGTCTTTGTAGGTTGGCCTACA	AATTTTTGTCAGTGATTCAGCATTGCAAGA	2.3

Note.— Long-range PCR conditions were as follows: enzyme and buffer, Advantage2 kit (Clontech) using manufacturer’s buffer; primer concentration, 1 μM each; cycling, 95°C for 1 min followed by 30 cycles of 95°C for 0.5 min, 68°C for 12 min.

^aProduct includes a 2.7-kb deletion proximal to WHT2943's P4/distal-P1 deletion; see table A3.