. 2002 Sep 20;71(4):906–922. doi: 10.1086/342928

Table A7.

Primer Pairs Used for PCR Amplification of Deletion Junctions^[Note]

Patient(s)	PrimerIdentificationNumbers	Forward Primer	Reverse Primer	Deletion-JunctionProduct Size(bp)
AMC0110	10821/6	GGCAAGTTTCACAATCTGCTG	CAAAGATGCAGGTACTGCTCTGC	1,534
WHT4396	10827/12088	TCTTGATCAATGCCTTGTGAAAT	CCACTGTAAGTCTGACGGGG	740
WHT2943^a	11189/90	ACTAAATGCCCACAGGAGAAAA	TTGTCATGGATATCCCATCCA	976
WHT3410^a	11075/115	GGTCTTCTCACGTAATCCCG	TGTCCTGGATGGTAATGCC	754
WHT3516, WHT3642, WHT4426, and WHT4486	10824/916^b	CAAAGATGCGGGTACTGCTCTAT	ATTGGCAAGTTTCATAATCTGCTA	1,537
WHT2825	11086/7	TGAGCATAAGTTTGTTTCGGG	CGAGGATGTCAATGTTTTGC	421

Note.— For PCR conditions, see sY1201 (GenBank).

We first used long-range PCR (table A8) and then, based on the sequence of the long-range PCR product, designed the primers shown in this table.

Annealing at 63°C.