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. 2014 Jun 18;307(8):C701–C709. doi: 10.1152/ajpcell.00134.2014

Fig. 1.

Fig. 1.

Influence of 29-kDa endoplasmic reticulum (ER) protein (ERp29) overexpression on whole cell epithelial Na+ channel (ENaC) expression in Madin-Darby canine kidney (MDCK) epithelial cells. A: MDCK cells stably expressing murine αβγ-ENaC and with doxycycline (Dox)-inducible expression of wt ERp29 or C157S ERp29 were treated for 8 h with Dox (50 ng/ml). B: MDCK cells stably expressing murine αβγ-ENaC were transiently transfected with control, wt ERp29, or C157S ERp29 plasmid. Whole cell lysates were prepared, and ERp29, HA-tagged α-ENaC, V5-tagged β-ENaC, γ-ENaC, and GAPDH (A) and ERp29 and GAPDH (B) expression was determined by immunoblotting. Immunoblots (IB) are representative of >3 independent experiments; those in A are juxtapositions taken from noncontiguous lanes of the same experimental gels. *P < 0.05 versus control.