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ERp29 promotes ENaC functional expression in Xenopus oocytes. Xenopus oocytes were injected with cRNA encoding murine αβγ-ENaC (0.33 ng/subunit) or 10 ng of ERp29 cRNA or coinjected with αβγ-ENaC and ERp29 cRNAs. Whole oocyte current at −100-mV holding potential that was sensitive to inhibition by 10 μM amiloride was determined by 2-electrode voltage clamp 28–48 h after injection. Values are means ± SE. *P < 0.05 (by t-test).
