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β-ENaC interacts with ERp29. MDCK αβγ-ENaC wt or C157S ERp29 cells were treated with Dox for 8 h. Whole cell lysates were prepared under nondenaturing conditions. ERp29 and its interacting proteins were precipitated using anti-ERp29, and V5-tagged β-ENaC associated with ERp29 was revealed by immunoblotting. Input lane represents 10% of the total protein subject to immunoprecipitation (IP). Data are representative of 3 independent experiments. *P = 0.032.
