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. 2014 Aug 19;111(8):1646–1656. doi: 10.1038/bjc.2014.457

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Copyright © 2014 Cancer Research UK

From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

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Metastatic PC3 cells upregulate Etn release on phorbol ester treatment. Cells were cultured and labelled with [1-³H]-Etn (Methods). After rinsing, cells were incubated for 3 h with serum-free basal () medium (+DMSO) or basal medium containing 1 μM TPA (▪) Media were recovered and GPEtn, PEtn and Etn resolved by ion-exchange chromatography (Methods). Total d.p.m. associated with each fraction were calculated and results for each metabolite expressed as % of total GPEtn+PEtn+Etn d.p.m. Results shown are mean values +/−s.d. (n=3) from a typical experiment. For PC3 cells, GPE, basal against TPA **P= 0.005; PE, basal against TPA, *P=0.04; Etn, basal against TPA ***P=0.0003.

Inline graphic — Metastatic PC3 cells upregulate Etn release on phorbol ester treatment. Cells were cultured and labelled with [1-³H]-Etn (Methods). After rinsing, cells were incubated for 3 h with serum-free basal () medium (+DMSO) or basal medium containing 1 μM TPA (▪) Media were recovered and GPEtn, PEtn and Etn resolved by ion-exchange chromatography (Methods). Total d.p.m. associated with each fraction were calculated and results for each metabolite expressed as % of total GPEtn+PEtn+Etn d.p.m. Results shown are mean values +/−s.d. (n=3) from a typical experiment. For PC3 cells, GPE, basal against TPA **P= 0.005; PE, basal against TPA, *P=0.04; Etn, basal against TPA ***P=0.0003.