Figure 7.

(A–C). Oleate decreases Etn release from PNT2C2, P4E6 and PC3 cell lines and increases GPEtn and PEtn release. Etn metabolites in media from 3-h time course experiments were resolved by ion-exchange chromatography as described in Methods. Total d.p.m. for each metabolite was calculated and individual metabolites expressed as a % of GPEtn+PEtn+Etn. Results are mean values +/−s.d. (n=6) from duplicate media analyses. OL against basal values, (A), ***P<0.0001, **P<0.001; (B),**P=0.0002, ***P<0.0001; (C), **P=0.006, ***P<0.0001.