Table 2.
Identification of Peptide Sequences of Recombinant Human α-gal A[Note]
| PredictedMolecularWeight | ObservedMolecularWeight | Sequence |
| 726.3973 | 726.36 | (R)FPHGI R(Q) |
| 764.3977 | 764.35 | (K)QGYQL R(Q) |
| 1,063.4454 | 1,063.42 | (R)QYCNH WR(N) + |
| 1,158.5505 | 1,158.54 | (K)LGFYE WTSR(L) |
| 1,286.6455 | 1,286.64 | (R)KLGFY EWTSR(L) * |
| 1,413.6659 | 1,413.67 | (R)TPTMG WLHWE R(F) |
| 1,429.6608 | 1,429.63 | (R)TPTMG WLHWE R(F) # |
| 1,495.7103 | 1,495.73 | (K)SILDW TSFNQ ER(I) |
| 1,534.8164 | 1,534.84 | (R)LQADP QRFPH GIR(Q) * |
| 1,951.0785 | 1,951.07 | (K)ALLQD KDVIA INQDP LGK(Q) * |
| 2,044.0758 | 2,044.07 | (K)GVACN PACFI TQLLP VKR(K) * + + |
| 2,058.1278 | 2,058.01 | (K)GVACN PACFI TQLLP VKRK(L) ** |
| 2,696.4657 | 2,996.44 | (K)ALLQD KDVIA INQDP LGKQG YQLR(Q) ** |
| 1,174.5499 | Not found | (K)LGYYE WTSR(L) @ |
| 1,302.6449 | Not found | (R)KLGYYE WTSR(L) @ |
Note.— Indicated are molecular weights of peptides predicted on the basis of the α-gal A cDNA sequence and the molecular weights obtained by mass spectrometry. Shown in the right column are the amino acid sequences that comply with the observed masses; parentheses indicate neighboring amino acids that are not part of the peptide. The last two predicted molecular weights are based on the putative edited sequence of the α-gal A mRNA sequence (see fig. 1a). Symbols: + = carboxyamidomethylcysteine(s); * = missed cleavage(s); # = methionine sulfoxide; @ = “Edited” peptide.