Figure 2.

Correlative light/EM analysis and identification of new boutons on electron micrographs. A, Confocal image of a stimulated preparation (CD8-GFP). The boxed region is enlarged (right), and new boutons are indicated by arrows. Scale bar, 5 μm. B, Electron micrograph showing a section from the boxed region shown in A. This plane shows three new boutons (1, 2, 3) formed around a cluster of synaptic boutons surrounded by the SSR (arrowheads). Scale bar, 2 μm. C, Close-ups of boutons 2 and 3 at the edge of the synaptic cluster (top), as well as bouton 1 (bottom).