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. 2014 Aug 6;34(32):10554–10563. doi: 10.1523/JNEUROSCI.5074-13.2014

Figure 8.

Figure 8.

The movement of vesicle and cytosolic markers is not enhanced upon stimulation. A, B, Syt-GFP fluorescence shows no significant recovery either in the absence or in the presence of stimulation (n = 5 nonstimulated, n = 6 stimulated central boutons; n = 5 nonstimulated, n = 8 stimulated terminal boutons). Central and terminal boutons show a similar recovery pattern. Dotted lines show Syn-GFP fluorescence (the same as in Fig. 7, shown for comparison). C, D, Cytosolic GFP shows a significant recovery either in the absence or in the presence of stimulation. In both cases, it exceeds the recovery of Syn-GFP fluorescence (dotted lines). Central and terminal boutons show a similar recovery pattern (central, n = 7 per condition; terminal, n = 8 per condition).