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. 2014 Aug 6;34(32):10729–10742. doi: 10.1523/JNEUROSCI.0539-14.2014

Figure 3.

Figure 3.

ZPK/DLK- and MKK4/MAP2K4-dependent mechanism and ZPK/DLK- and MKK4/MAP2K4-independent mechanism contribute to elimination of excess facial MNs during development. A, ZPK/DLK-deficient (zpk/dlktp/tp) and CNS MKK4/MAP2K4-deficient [mkk4flox/flox Tg(nes::cre)+/−] embryos and their littermate controls were obtained from timed-pregnant mice, and serial cross-sections of the brainstem were double immunolabeled for Islet-1,2 and cleaved Caspase-3 (arrows and arrowheads). Facial nuclei are delineated by a dotted circle. Representative cleaved Caspase-3-positive cells (arrowheads in low-power micrographs) and their nuclei are magnified in color and inverted insets, respectively. Scale bars: 100 μm; insets, 10 μm. B, Quantitative results of facial MNs and cleaved Caspase-3-positive cells of ZPK/DLK-deficient embryos (tp/tp), wild-type littermates (+/+), and those of heterozygous (+/flox) and homozygous (flox/flox) floxed MKK4/MAP2K4 Nestin–Cre [Tg(Nes::Cre)+/−] embryos from E14.5 to E18.5. *p < 0.05, ***p < 0.001, and not significant (NS) compared with the indicated pair or with littermate controls (unpaired t test). At least four facial nuclei were counted in each dataset.