Figure 8.

Retrograde labeling of axotomized facial MNs with fluoro-ruby. Fluoro-ruby was applied to the proximal end of the transected nerve, and the facial nuclei were analyzed at 24 h after axotomy. Almost all facial MNs, except for most MNs in the VM (circumscribed by dots in the nuclei) and DA (arrows) subnuclei, were similarly labeled with fluoro-ruby regardless of the expression of either ZPK/DLK or MKK4/MAP2K4. Nevertheless, note that acute reduction in ChAT immunoreactivity in axotomized fluoro-ruby-positive MNs was not observed in ZPK/DLK-deficient and CNS MKK4/MAP2K4-deficient mice at 24 h after axotomy. Scale bar, 100 μm.