Figure 3.

Fluorescent images of COS-7 cells expressing HA-tagged CRYM proteins. Nuclei are counterstained with DAPI (blue). Cells representative of six independent experiments are shown at a magnification of 60×. Subsequently, the numbers of cells were counted in 8–10 randomly selected fields (60×) using fluorescence microscopy (Nikon) at 24 h, 48 h, 72 h, and 96 h, respectively. A, Wild-type (WT) CRYM protein (green) localizes to cytoplasm diffusely throughout the time course of the experiments. B, The X315Y mutant with an extended C-terminal tail leads to vacuolated cytoplasmic distribution. The K314T mutant protein is localized predominantly in the perinuclear area.