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. 2014 Aug 29;289(42):28808–28815. doi: 10.1074/jbc.M114.583534

FIGURE 3.

FIGURE 3.

GABABRs increase GS expression levels. A, COS-7 cells expressing GS, GS/R2, GS/β3 and GS/R1/R2 were immunoblotted with R1, R2, β3, GS, and actin antibodies. The levels of GS expression were normalized to cells expressing this protein alone. Data represent mean ± S.E. (p < 0.01; ANOVA, n = 4). B, cells expressing GS, GS/R2 and GS/R2ΔCT were immunoblotted with R2 and GS antibodies. The ratio of GS:R2 immunoreactivity was determined and normalized to cells expressing wild type R2. Data represent mean ± S.E. (p < 0.01; unpaired t test, n = 3). *, significantly different from control (p < 0.001).