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. 2014 Aug 28;289(42):28846–28855. doi: 10.1074/jbc.M114.585117

FIGURE 1.

FIGURE 1.

DNA binding activity of MsRecO is stimulated independently by zinc and RecR. A, isotherm showing DNA binding measured by change in fluorescence anisotropy of FAM-dT15 (5 nm) upon titration by EcRecO (EcO; gray) and MsRecO (MsO; black) in buffer A at 50 mm NaCl in the presence (square) or absence (circle) of 8 μm MsRecR without (open) and with 10 μm Zn(OAc)2 (solid). B, similar measurements of DNA binding by EcRecO (gray) and MsRecO (black) in the presence of cognate RecR (5 μm) in buffer B with 100 mm NaCl.