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DmlA unfolds at a higher temperature in the presence of d-malate and 3-isopropylmalate, but not l-(+)-tartrate. Tm was measured by differential scanning fluorometry of the apo form and in the presence of 1 mm d-malate, 3-isopropylmalate, and l(+)-tartrate. The ΔTm values represent the difference in Tm between the protein in the presence of the specified substrate and the apoprotein.
