FIGURE 6.

Kinetic characterization of DmlA on various substrates. The k_obs values were measured for six substrates at different concentrations with fixed concentrations of NAD⁺ (see “Experimental Procedures” for concentrations) and Mn²⁺ (5 mm). The kinetic parameters were calculated from the data by fitting to the Michaelis-Menten equation (Equation 2) (A–C and F) and by estimating the k_cat/K_m parameter from the linear part of the activity-substrate curve (D and E). The calculated parameters are reported in Table 1.