. 2014 Sep 4;289(42):29112–29122. doi: 10.1074/jbc.M114.571679

TABLE 1.

RT-PCR primer list

UCP3, uncoupling protein 3; MCAD, medium-chain acyl-CoA dehydrogenase; Cyt-c, cytochrome c; aP2, adipocyte protein 2; HSL, hormone-sensitive lipase; SCD1, stearoyl-CoA desaturase 1.

Target	Forward	Reverse
β-Actin	`GGCTGTATTCCCCTCCATCG`	`CCAGTTGGTAACAATGCCATGT`
TNFα	`CCCTCACACTCAGATCATCTTCT`	`GCTACGACGTGGGCTACAG`
aP2^a	`GGCGTGACTTCCACAAGAGTTTA`	`GCCTCTTCCTTTGGCTCATG`
PPARγ^a	`CAAGAATACCAAAGTGCGATCAA`	`GAGCTGGGTCTTTTCAGAATAATAAG`
PGC-1α^a	`AACCACACCCACAGGATCAGA`	`TCTTCGCTTTATTGCTCCATGA`
UCP3	`CAACGGTTGTGAAGTTCCTG`	`CTCTGTGCGCACCATAGTCA`
HSL	`CTGCTGACCATCAACCGAC`	`CGATGGAGAGAGTCTGCA`
MCAD^a	`GCCAAGATCTATCAGATTTATGAAGGT`	`AGCTATGATCAGCCTCTGAATTTGT`
PPARα^a	`ACAAGGCCTCAGGGTACCA`	5'`GCCGAAAGAAGCCCTTACAG`
CPT1b^b	`ATCATGTATCGCCGCAAACT`	`CCATCTGGTAGGAGCACATGG`
PDK4^b	`CCGCTTAGTGAACACTCCTTC`	`TCTACAAACTCTGACAGGGCTTT`
LXRa^b	`AGCGTCCATTCAGAGCAAGT`	`CCCTTCTCAGTCTGCTCCAC`
Scd1^b	`CCTCCTGCAAGCTCTACACC`	`CAGCCGAGCCTTGTAAGTTC`
Sirt1^c	`GCTGACGACTTCGACGACG`	`TCGGTCAACAGGAGGTTGTCT`
Cyt-c^d	`GCAAGCATAAGACTGGACCAAA`	`TTGTTGGCATCTGTGTAAGAGAATC`

^a From Pan et al. (45).

^b From Kleiner et al. (15).

^c From Wang and Seed (46). PrimerBank ID: 9790229a1.

^d From Seale et al. Ref. 47.