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. 2014 Sep 2;289(42):29180–29194. doi: 10.1074/jbc.M114.576561

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Interactions between BLOS1 and SNX2/TSG101. A, FLAG-tagged BLOS1 co-precipitates with Myc-SNX2 but not with Myc-SNX1. The FLAG empty vector serves as a negative control. IP, immunoprecipitation; WB, Western blotting. B, GST-BLOS1 pulls down His-SNX2 but not His-SNX1. GST alone serves as a negative control. C, BLOS1 interacts with TSG101 in the yeast two-hybrid system. Full-length TSG101 is used as a bait (with binding domain, pGBKT7) to prey BLOS1 (with activation domain, pGADT7). The left panel shows the growth of yeast on synthetic defined (SD) medium lacking leucine and tryptophan. The right panel shows the growth of yeast on SD medium lacking adenine, histidine, leucine, and tryptophan. D, GST-BLOS1 pulls down His-TSG101, although GST alone does not. The blots shown here are representative ones from three independent experiments.