FIGURE 3.

Co-localization of BLOS1 with SNX2/TSG101 and endosomal targeting of EGF is delayed in stable BLOS1 knockdown HeLa cells. A, GFP-BLOS1 partially co-localizes with endogenous SNX2 and TSG101. The co-localization ratios were calculated by Pearson's correlation. B, representative images at different time points upon EGF (red) treatment in BLOS1-KD cells and normal control cells (NC). Endogenous EEA1 is labeled in green. Scale bar, 10 μm. C, co-localizations of EGF and EEA1 are represented by Mander's overlap coefficient K1. Data collected are from 50 cells and calculated as mean ± S.E. *, p < 0.05.