FIGURE 5.

Deposition of soluble Ser³¹-Gln⁶⁷ and Phe³³-Lys⁶⁵ peptide onto its respective fibrils (seeds). A, reaction mixtures of Ser³¹-Gln⁶⁷ (50 or 10 μm) plus sonicated f(Ser³¹-Gln⁶⁷) (2.5 or 5 nmol/100 μl) (left panel), Gal-7 molecule (50 or 10 μm) plus sonicated f(Ser³¹-Gln⁶⁷) (5 nmol/100 μl) (middle panel), and Phe³³-Lys⁶⁵ (50 or 10 μm) plus sonicated f(Phe³³-Lys⁶⁵) (2.5 or 5 nmol/100 μl) (right panel) were incubated for 3, 6, and 18 h or 30 and 48 h in 50 mm citrate buffer, pH 2.0, containing 100 mm NaCl, and then the ThT fluorescence intensities were measured. B, electron micrographs of reaction mixtures composed of f(Ser³¹-Gln⁶⁷) (5 nmol/100 μl) plus Ser³¹-Gln⁶⁷ (50 μm) (left panel) and f(Phe³³-Lys⁶⁵) (5 nmol/100 μl) plus Phe³³-Lys⁶⁵ (50 μm) (right panel) after 18 h of incubation. Bars; 500 nm.