FIGURE 6.

EB1 knockdown reduces the fusion of autophagic vesicles and lysosomes. A, NPC TW02 cells were pretreated with control siRNA (siC) or EB1 siRNA for 48 h and then treated with/without poly(dA-dT) for 6 h. The cells were fixed and stained with anti-LC3 and anti-LAMP1 antibodies and then observed under confocal fluorescence microscopy. B, HeLa cells were prepared and observed as described in A. Scale bar, 20 μm.