TABLE 2.
Primary antibodies
Indices (i) and (j) correspond to antibodies and PLB species, respectively, in Eq. 1. Either AbA1 or Ab2D12 were used in both Figs. 1 and 2 to emphasize the robustness of the assay. Cell Signaling Technology reports that the Ab8496 epitope has 100% sequence homology with pig and human, thus excluding E2D and K27N.
| Antibody | Epitope | Ab (i) | PLB selectivity (j) | Reactivity | Dilution | Company |
|---|---|---|---|---|---|---|
| AbA1 | 7–16 | AbU (1) | U-PLB (1) | Mouse | 1:1000 | Millipore |
| Ab2D12 | 9–17 | AbU (1) | U-PLB (1) | Mouse | 1:600 | Abcam |
| Ab12963 | 11–21 | Ab16 (2) | P16-PLB and 2P-PLB (2) | Goat | 1:1000 | Santa Cruz Biotechnology |
| Ab17024-R | 12–22 | Ab17 (3) | P17-PLB and 2P-PLB (3) | Rabbit | 1:2000 | Santa Cruz Biotechnology |
| Ab8496 | proprietary | Ab2P (4) | 2P-PLB (4) | Rabbit | 1:2000 | Cell Signaling Technology |