Skip to main content
. 2014 Sep 5;289(42):29420–29436. doi: 10.1074/jbc.M114.603068

FIGURE 5.

FIGURE 5.

RNF34 co-immunoprecipitates with brain GABAARs. A and B, various antisera to the γ2 GABAAR subunit, two raised in rabbit (Rb γ2 and Rb γ2(1–29), in A) and one in guinea pig (GP γ2, in B) co-precipitate RNF34 (42 kDa) with GABAARs from rat forebrain membrane extracts. GABAARs in the precipitate were revealed by the presence of γ2 (45 kDa) and α1 (50 kDa) GABAAR subunits. C and D, antisera to the α1 GABAAR subunit raised in rabbit (Rb α1, in C) or guinea pig (GP α1, in D) co-precipitate RNF34 and GABAARs, as shown by the presence of RNF34, γ2, and α1 subunits in the precipitate. In all panels, the presence of RNF34 and GABAARs in the precipitate was detected by immunoblotting with Rb anti-RNF34, Ms anti-γ2IL, Ms anti-α1, with the exception of α1 in D where Rb anti-α1* was used.