FIGURE 2.
The chimera bBidomain contains a (GGGS)5 linker. A, partial protein sequence alignment of human inducible NOS (iNOS), human endothelial NOS (eNOS), rat neuronal NOS (nNOS) and bBiDomain. Sequences were manually aligned based on crystal structure comparison of oxygenase domain using the homologous residues indicated by (single asterisk) and the FMN-binding domains are underlined. bBidomain oxygenase and FMN domain sequences are highlighted red and yellow, respectively. B, model for electron transfer from NADPH to bBiDomain.