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. 2014 Sep 6;289(42):29437–29445. doi: 10.1074/jbc.M114.595165

FIGURE 5.

FIGURE 5.

Isothermal titration calorimetry data of l-Arg titrated into bsNOS in 50 mm KPi, pH 7.4, at 25 °C. A, raw isothermal titration calorimetry data plotted as heat generated versus time for one of three trials. B, integrated and concentration-normalized heat evolved for one of three trials at each injection. A one-site model was used to generate the thermodynamic values for l-Arg binding KD (or 1/KA) = 5.6 ± 0.1 μm, ΔH = −7.73 ± 0.02 kcal/mol and TΔS = −0.047 ± 0.002 kcal/mol based on the average and S.E.M. of three separate trials.