TABLE 2.

Chemical name and calculated K_S for substrate/inhibitor to active site of bsNOS, bsNOS in the presence of a 10 molar excess YkuN and bBiDomain

Error represents the error associated with the fit curve used to calculate the K_S. Values not determined (n.d.) are indicated.

Compound	Chemical name	bsNOS	bsNOS + 10x YkuN	bBiDomain
		KS, μm	KS, μm	KS, μm
l-Arg	(S)-2-Amino-5-guanidinopentanoic acid	0.8 ± 0.1 (8)	6.5 ± 0.2	16 ± 2
NOHA	N-Omega-hydroxy-l-arginine	3.2 ± 0.5	2.2 ± 0.1	13 ± 5
l-NNA	N-Omega-nitro-l-arginine	1.3 ± 0.1 (8)	4.9 ± 0.9	24 ± 7
l-NAME	N-Omega-nitro-l-arginine methyl ester	66 ± 46	n.d.	n.d.
ITU	Ethylisothiourea	9.3 ± 1.2	n.d.	n.d.
7-NI	7-Nitroindazole	3.5 ± 0.4	6.8 ± 0.8	27 ± 4
7-NI-Br	3-Bromo-7-nitroindazole	12 ± 1	n.d.	n.d.
1	6-({[(3R,5S)-5-{[(6-Amino-4-methylpyridin-2-yl)methoxy]methyl}pyrrolidin-3-yl]oxy}methyl)-4-methylpyridin-2-amine	4.4 ± 0.1 (8)	n.d.	n.d.
2	6,6′-{[(2S,3S)-2-Aminobutane-1,3-diyl]bis(oxymethanediyl)}bis(4-methylpyridin-2-amine)	1.1 ± 0.1 (8)	n.d.	n.d.